Syzygium rubicundum
Seed germination try very staggered in both absolute (Stacy, Harischandran, and you may Gunatilleke, when you look at the push) and you may greenhouse setup, extending over >4 mo. In the a hundred d shortly after growing (whenever germination is actually ?95% complete), the newest rates out of seeds germinated were average, ranging around the service away from 21 so you can forty-two% from sown seed (indicate = 36%; pooled round the maternal woods; Fig. 3a). With the exception of brand new oddly higher germination rate noticed towards nearest-next-door neighbor medication from the Forest no. 3 (83.3%), the partnership between crossing range and you will vegetables germination are nearly the same compared to that having fruits set for which kinds (Fig. 3a). Including most of the analysis affairs, indicate seed products germination wasn’t rather various other certainly one of services, nor certainly one of maternal woods (Desk 3A). Excluding the solitary outlier didn’t change the Aong hands-pollination providers, height indicate vegetables germination coincided which have height fruit set in the an outcrossing point of 1–dos kilometres and you may is almost just like brand new indicate germination off open-pollinated seed products (Fig. 3a).
Shorea cordifolia
Germination of experimental seeds was rapid and fairly even across treatments (mean = 62%; range: 55–72% at 45 d; Fig. 3b). A steady increase in mean seed germination with outcrossing distance is apparent, but not significant (Fstep one,9 = 1.02, P < 0.34, R 2 = 0.40; Fig. 3b). Mean seed germination was not significantly different among treatments, nor among maternal trees (Table 3B). Interestingly, mean seed germination for all hand-pollination treatments exceeded that of open-pollinated seeds (Fig. 3b). While the reason for this observation is not known, the latter estimates were based on much larger sample sizes and therefore may be more robust.
Seedling survivorship and you may development
Because of attrition from 1 life stage to a higher, sample types of a few treatment classes was in fact low having rates regarding seedling survivorship and you may progress, which may have impeded analytical evaluation. Zero high results of either crossing medication or maternal tree was indeed discovered having both scale in either varieties (Desk 3). Excluding selfed cures seedlings from S. rubicundum (N = 2), the newest imply survivorship speed away from seedlings throughout their first 12 months is actually high (>80%) for everyone service off both variety. For both species, level indicate seedling survivorship coincided on distant within this-forest therapy.
For S. rubicundum, mean seedling peak on step one yr postgermination increased having outcrossing distance, peaking within a keen intermate point of just one–2 km, and dropping some towards the longest length crosses (Fig. 4a). Having Sh. cordifolia, suggest seedling height increased steadily with outcrossing length, peaking at longest-range get across (Fig. 4b). For variety, within-therapy type during the seedling level is large (Fig. 4). To have S. rubicundum, version in seedling height will likely be informed me, at the very least simply, by staggered seeds germination.
Collective fitness
For each species, the cumulative fitness of each treatment class was calculated as the standardized product of the four fitness measures (see Fig. 5). For both species, the distribution of cumulative fitness with respect to crossing distance was similar to that for fruit set (Fig. 5). For S. rubicundum, variation in cumulative fitness among treatments was highly significant (Table 3A); mean cumulative fitness of the distant within-forest treatment was 4–25 times the means for other hand-pollination treatments and twice that of open-pollinated flowers. The relationship between outcrossing distance and cumulative fitness was nearly significant (quadratic regression: Fdos,10 = 3.84, P = 0.056). Mean cumulative fitness (relative) was not significantly different between the distant between-forest treatment (mean = 0.07) and all within-forest outcrossed classes combined (mean = 0.24, Fstep 1,twelve = 1.69, P = 0.219), due to the strong near-neighbor effect on cumulative fitness.